The Accelerating Rotating Rod Assay or Rotarod Test: A Method to Test Motor Coordination and Learning in Mice
Published: April 30, 2023
Abstract
Source: Ferro, A., et al. Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function. J. Vis. Exp. (2017).
In this video, we perform the accelerating rotating rod or rotarod test to assess motor coordination and learning in mouse subjects.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Accelerating Rotarod Acclimate subjects to the assay room 10 min prior to the start of the trial. During this acclimation period, prepare the rotarod apparatus, cleaning each lane of the apparatus with 30% ethanol. Open the rotarod software and set start settings to 4 rpm, acceleration settings to 5 min, and f…
Divulgaciones
The authors have nothing to disclose.
Materials
| ANIMALS | |||
| Wildtype mice | The Jackson Laboratory | 1800 | |
| EQUIPMENT | |||
| Rotallion Rotarod | PPP&G | ||
| SOFTWARE | |||
| 3P&G Rota-Rod v3.3.3 (rotarod software) | PPP&G |
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The Accelerating Rotating Rod Assay or Rotarod Test: A Method to Test Motor Coordination and Learning in Mice. J. Vis. Exp. (Pending Publication), e20043, doi: (2023).