13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement

13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement (Video) | JoVE

Published: May 19, 2023

doi:

10.3791/200017

Tenzin Tseyang*¹, Jonathan Valeros*¹, Paula Vo*¹, Jessica B. Spinelli²

¹Program in Molecular Medicine,University of Massachusetts Chan Medical School, ²Cancer Center,University of Massachusetts Chan Medical School

Tags

Keywords: 13C4-aspartate 13C5-glutamine Stable Isotope Tracing LC-MS Analysis SDH Activity Measurement 143B Human Osteosarcoma Cells Cell Culture Metabolite Extraction Vacuum Concentration Liquid Chromatography Mass Spectrometry

Play Video

PDF

DOI

Citar este artículo

¹³C₄-Aspartate and ¹³C₅-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement. J. Vis. Exp. (Pending Publication), e200017, doi:10.3791/200017 (2023).

13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement (Video) | JoVE

Tags

Play Video

Citar este artículo

View Video

✖

To prove you're not a robot, please enter the text in the image below