JOVE JOURNAL

Bioquímica

Showing 13 - 24 in 776 videos.

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Journal (Biochemistry)

Modeling Phosphoenolpyruvate in Enolase Enzyme from Mycobacterium tuberculosis

Shaileshanand Jha*¹, Sucharita Bose*², Kutti R. Vinothkumar¹

¹National Centre for Biological Sciences,Tata Institute of Fundamental Research, ²Institute for Stem Cell Science and Regenerative Medicine

Journal (Biochemistry)

Modeling Ligands into Maps Derived from Electron Cryomicroscopy

Shaileshanand Jha*¹, Sucharita Bose*², Kutti R. Vinothkumar¹

¹National Centre for Biological Sciences,Tata Institute of Fundamental Research, ²Institute for Stem Cell Science and Regenerative Medicine

Journal (Biochemistry)

Preparation of Quantum Dots (QDs) Nanobeads for QDNB-Based Lateral Flow Immunoassay

Lingzhi Fan*¹, Yue Luo*², Wannian Yan¹, Huanxing Han³, Pengfei Zhang¹

¹Department of Central Laboratory, Shanghai Skin Disease Hospital, School of Medicine,Tongji University, ²Department of Dermatology, Shanghai Skin Disease Hospital, Institute of Dermatology, School of Medicine,Tongji University, ³Department of Pharmacy, Changzheng Hospital,Naval Medical University

Journal (Biochemistry)

QDNB-Antibody Conjugates Preparation Using One-Step Emulsion Evaporation Method

Lingzhi Fan*¹, Yue Luo*², Wannian Yan¹, Huanxing Han³, Pengfei Zhang¹

¹Department of Central Laboratory, Shanghai Skin Disease Hospital, School of Medicine,Tongji University, ²Department of Dermatology, Shanghai Skin Disease Hospital, Institute of Dermatology, School of Medicine,Tongji University, ³Department of Pharmacy, Changzheng Hospital,Naval Medical University

Journal (Biochemistry)

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Lingzhi Fan*¹, Yue Luo*², Wannian Yan¹, Huanxing Han³, Pengfei Zhang¹

¹Department of Central Laboratory, Shanghai Skin Disease Hospital, School of Medicine,Tongji University, ²Department of Dermatology, Shanghai Skin Disease Hospital, Institute of Dermatology, School of Medicine,Tongji University, ³Department of Pharmacy, Changzheng Hospital,Naval Medical University

Journal (Biochemistry)

Lateral Flow Immunoassay Strips Preparation, Assay Operation and its Quantitative Evaluation

Lingzhi Fan*¹, Yue Luo*², Wannian Yan¹, Huanxing Han³, Pengfei Zhang¹

¹Department of Central Laboratory, Shanghai Skin Disease Hospital, School of Medicine,Tongji University, ²Department of Dermatology, Shanghai Skin Disease Hospital, Institute of Dermatology, School of Medicine,Tongji University, ³Department of Pharmacy, Changzheng Hospital,Naval Medical University

Journal (Biochemistry)

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro

Craig McBeth¹, David Brown², Pawel Pokorski², Lydia Lei², Vicki Stone²

¹School of Health and Life Sciences,Teesside University, ²Institute of Biochemical Chemistry, Biophysics, and Bioengineering,Heriot-Watt University

Journal (Biochemistry)

OPA Assay for Accurate Glutathione Detection in Cells

Craig McBeth¹, David Brown², Pawel Pokorski², Lydia Lei², Vicki Stone²

¹School of Health and Life Sciences,Teesside University, ²Institute of Biochemical Chemistry, Biophysics, and Bioengineering,Heriot-Watt University

Journal (Biochemistry)

Quantifying the Level of 8-oxo-dG Using ELISA Assay to Evaluate Oxidative DNA Damage in MCF-7 Cells

Liu Nian*^1,2, Xiaohua Li*³, Jiahui Du¹, Song-Bai Liu^1,2

¹Suzhou Key Laboratory of Medical Biotechnology,Suzhou Vocational Health College, ²Department of Chemistry and Life Sciences,Suzhou University of Science and Technology, ³Department of Thyroid and Breast Surgery,Wuzhong People’s Hospital of Suzhou City

Journal (Biochemistry)

Quantifying DNA Oxidative Damage in MCF-7 Cells After Hydrogen Peroxide Treatment

Liu Nian*^1,2, Xiaohua Li*³, Jiahui Du¹, Song-Bai Liu^1,2

¹Suzhou Key Laboratory of Medical Biotechnology,Suzhou Vocational Health College, ²Department of Chemistry and Life Sciences,Suzhou University of Science and Technology, ³Department of Thyroid and Breast Surgery,Wuzhong People’s Hospital of Suzhou City

Journal (Biochemistry)

Culturing the Endothelial Cell Line in 96-Well Plates for the Calcium Mobilization Assay

Jacob T. DeRousse^1,2,3, Chris Dockendorff^1,2

¹Function Therapeutics, ²Department of Chemistry,Marquette University, ³School of Medicine,Washington University

Journal (Biochemistry)

Enhanced Plate Reader Assay for Calcium Mobilization in Endothelial Cells

Jacob T. DeRousse^1,2,3, Chris Dockendorff^1,2

¹Function Therapeutics, ²Department of Chemistry,Marquette University, ³School of Medicine,Washington University

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