Instituto de Salud Carlos III 3 articles published in JoVE Biology Quantifying Fitness Costs in Transgenic Aedes aegypti Mosquitoes Adeline E. Williams1,2, Irma Sanchez-Vargas1, Lindsay E. Martin2,3, Ines Martin-Martin2,4, Susi Bennett1, Ken E. Olson1, Eric Calvo2 1Center for Vector-borne Infectious Diseases, Department of Microbiology, Immunology, and Pathology, Colorado State University, 2Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 3Department of Biological Sciences, Vanderbilt University, 4National Center for Microbiology, Instituto de Salud Carlos III The present protocol describes how to measure common life parameter data in Aedes aegypti mosquitoes, including fecundity, wing size, fertility, sex ratio, viability, development times, male contribution, and adult longevity. These measurements can be used to assess the fitness of transgenic mosquitoes. Biology Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays Juan Diego Hernández-Camacho1,2, Cristina Vicente-García1, Ana Sánchez-Cuesta1,2, Daniel J. M. Fernandez-Ayala1,2, Jaime J. Carvajal1, Plácido Navas1,2 1Centro Andaluz de Biología del Desarrollo, CSIC-UPO-JA, Universidad Pablo de Olavide, 2CIBERER, Instituto de Salud Carlos III Here, we describe a detailed method for mitochondria isolation from mouse skeletal muscle and the subsequent analysis of respiration by Oxygen Consumption Rate (OCR) using microplate-based respirometric assays. This pipeline can be applied to study the effects of multiple environmental or genetic interventions on mitochondrial metabolism. Medicine Myocardial Infarction by Percutaneous Embolization Coil Deployment in a Swine Model Daina Martínez-Falguera*1,2, Edgar Fadeuilhe*3, Albert Teis3, Julia Aranyo3, Raquel Adeliño1,3, Felipe Bisbal3,4, Oriol Rodriguez-Leor3,4, Carolina Gálvez-Montón1,3,4 1ICREC Research Program, Germans Trias i Pujol Health Research Institute (IGTP), 2Faculty of Medicine, University of Barcelona (UB), 3Heart Institute (iCOR), Germans Trias i Pujol University Hospital, 4CIBER Cardiovascular, Instituto de Salud Carlos III Myocardial infarction (MI) animal models that emulate the natural process of the disease in humans are crucial to understanding pathophysiological mechanisms and testing the safety and efficacy of new emergent therapies. Here, we describe an MI swine model created by deploying a percutaneous embolization coil.
