FRET-Based Method to Monitor LRRC8 Channel Activity Without Electrophysiology

Electrophysiology is the gold standard for investigating ion channel activity. However, there are plenty of alternative approaches, including optical methods. Here, we describe a method to monitor the activity of the leucine-rich repeat containing 8 channel (LRRC8)-formed anion channels using an inter-subunit Förster resonance energy transfer (FRET)-based method.