Histone Proteomic Analysis and Post-Translational Modification Characterization

Histone Proteomic Analysis and Post-Translational Modification Characterization

January 12, 2024

使用液相色谱法、捕获离子淌度谱法和飞行时间质谱法进行组蛋白修饰筛选

配置和校准用于蛋白质组学分析的 TIMS 软件界面

用于蛋白水解组蛋白肽分析的LC-TIMS-PASEF-ToF MS/MS方法

Meiby Fernandez-Rojas¹, Cassandra N. Fuller¹, Lilian Valadares Tose¹, Matthew Willetts², Melvin A. Park², Natarajan V. Bhanu³, Benjamin A. Garcia³, Francisco Fernandez-Lima^1,4

¹Department of Chemistry and Biochemistry,Florida International University, ²Bruker Daltonics Inc., ³Department of Biochemistry and Molecular Biophysics,Washington University School of Medicine, ⁴Biomolecular Sciences Institute,Florida International University

Summary

基于液相色谱、俘获离子淌度谱和飞行时间质谱（LC-TIMS-ToF MS/MS）的分析工作流程，用于基于主要参数（保留时间 [RT]、碰撞截面 [CCS] 和准确的质荷比 [m/z] 比）对组蛋白修饰和鉴定进行高可信度和高度可重复性的“自下而上”分析。

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