De-Sialylated Monocyte-Derived-DCs from Isolated PBMCs Using Sialidase Treatment
1Associate Laboratory i4HB – Institute for Health and Bioeconomy, NOVA School of Science and Technology,Universidade NOVA de Lisboa, 2UCIBIO – Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology,Universidade NOVA de Lisboa, 3LAQV and REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia,Universidade NOVA de Lisboa, 4Department of Microbiology and Immunology,Albert Einstein College of Medicine, 5Department of Oncology,Albert Einstein College of Medicine, 6Stemmatters, Biotecnologia e Medicina Regenerativa SA, Parque de Ciência e Tecnologia Avepark,Zona Industrial da Gandra, 7CDG & Allies – Professionals and Patient Associations International Network (CDG & Allies – PPAIN), Department of Life Sciences, NOVA School of Science and Technology,Universidade NOVA de Lisboa
Summary
提出了一种独特的、全面的方案，使用唾液酸酶处理从分离的外周血单核细胞 （PBMC） 中产生去唾液酸化的人单核细胞来源的树突状细胞 （mo-DC）。此外，还描述了评估mo-DCs的表型和功能表征以及评估唾液酸酶处理如何改善mo-DCs成熟水平的方法。
