Encyclopedia Of Experiments

Neuronal Culture Techniques

This collection features a variety of neuronal culture techniques, ranging from basic neuron cultures to advanced protocols for generating neural progenitors. It covers specific neuron cultures that employ three-dimensional scaffolds or microfluidics, and differentiation into brain organoids and networks. Techniques also encompass co-cultures to study neuron-glia interactions and synapse formation, among other approaches for exploring neuronal behavior and neurogenesis in vitro.

Encyclopedia Of Experiments

Neuronal Culture Techniques

This collection features a variety of neuronal culture techniques, ranging from basic neuron cultures to advanced protocols for generating neural progenitors. It covers specific neuron cultures that employ three-dimensional scaffolds or microfluidics, and differentiation into brain organoids and networks. Techniques also encompass co-cultures to study neuron-glia interactions and synapse formation, among other approaches for exploring neuronal behavior and neurogenesis in vitro.

Sections

Primary Neuronal Culture

Co-culture and Interaction Studies

Advanced Neuronal Models

Primary Neuronal Culture

Co-culture and Interaction Studies

Advanced Neuronal Models

Primary Neuronal Culture

A Technique to Culture Mouse Hippocampal Neurons

Generating a Low-Density Primary Neuron Culture From Frozen Embryonic Neurons

Culturing Fluorescent Excitatory Neurons from a Transgenic Mouse Pup Brain

Generating a Primary Culture of Astrocytes from a Rat Pup Brain

Isolation of Microglia from the Cortex of an Adult Mouse Brain

A Simple Technique to Isolate and Culture Murine Astrocytes

Differentiating Human Induced Pluripotent Stem Cells into Neurons on Micro-Electrode Arrays

Isolation and Culture of Primary Neurons and Glia from a Rat Urinary Bladder

Compartmentalized Primary Murine Neuron Culture Using Plastic Microfluidic Chips

Isolation and Culture of Mouse Primary Cerebellar Granule Neurons

Culturing Murine Spiral Ganglion Neuron Explants on Multielectrode Arrays

Generation of Primary Retinal Ganglion Cell Cultures From Zebrafish Embryos

Generation of Neuronal Cells from Blood-Derived Pluripotent Stem Cells

Isolation and Culture of Microglia from Rat Brains using Serum-Free Media

Isolation and Culturing of Adult Neural Stem Cells from the Mouse Subcallosal Zone

Generating Neural Progenitors from Mouse Embryonic Stem Cells by the Hanging Drop Method

Obtaining a Mixed Oligodendrocyte and Astrocyte Culture From Adult Mouse Neural Stem Cells

Isolating Induced Neural Progenitor Cell Colonies Derived From Adult Human Fibroblasts

Generating an Ultra-Low-Density Neuronal Culture Using a High-Density Neuronal Feeder Layer

Differentiation of a Human Neuroblastoma Cell Line into Mature Neurons

Isolation of Cells from Sensory Ganglia

Dissociating and Culturing Neurons from Hippocampal Tissue Samples

Culturing and Maintaining Dopaminergic Neurons from Mouse Embryonic Brain Tissue

Preparation of Colliculo-Thalamocortical Slices from a Mouse Pup Brain

A Technique for Harvesting and Dissociating Dorsal Root Ganglia for Neuronal Cultures

Isolating and Culturing Murine Cerebellar Granular Neuron Progenitors

Isolation of Neural Stem Progenitor Cells from the Spinal Cord Periventricular Region of an Adult Rat

Isolating Fluorescently Labeled Neurons from a Murine Brain

Obtaining a Single-Cell Suspension from Mouse Hippocampal Tissue

Preparation of Collagen-Coated Compartmented Culture Dishes for Neuronal Cell Culture

Preparing a Single-Cell Suspension of Immune Cells from Murine Brain Tissue

Isolating Vestibular Ganglion Neurons From a Mouse Inner Ear

Isolating Murine Primary Oligodendrocytes Using Immunomagnetic Beads

Isolating and Culturing Oligodendrocyte Precursor Cells from Mouse Pup Brain Cortex

Isolation of Schwann Cells from Mouse Spinal Roots

Generating Dorsal Root Ganglion Explant and Dissociated Cell Culture

Obtaining Horizontal Mouse Hippocampal Brain Slices

Generation of Tilted Amygdala Slices from a Mouse Brain

Generating a Coronal Brain Slice Exposing the Amygdala Regions from a Mouse Brain

Isolating Microglia from Mouse Brain Demyelinating Lesions Using Magnetic Activated Cell Sorting

Isolation of Multiple Cell Types from a Mouse Brain by Mechanical Homogenization

Isolating Immune Cells from Mouse Choroid Plexuses

Obtaining a Mixed Glial Cell Culture from a Neonatal Mouse Brain

Isolating Cell Populations from Brain Tissue Using Fluorescence-Activated Nuclei Sorting

Obtaining Various Neural Cell Populations from a Rat Hippocampus

Isolation of Neurons from Caenorhabditis elegans via Fluorescence-Activated Cell Sorting

Obtaining Cerebellum Slices from an Embryonic Chick Brain

Isolating Murine Retinal Ganglion Cells Using Fluorescence-Activated Cell Sorting

Preparation of Mouse Vomeronasal Organ for Vibratome Sectioning

Isolation and Culturing of Cells from Mouse Embryo Dorsolateral Telencephalons

Establishing a Primary Dorsal Root Ganglia Cell Culture from a Rat Spinal Column

Isolation and Culture of Primary Embryonic Mouse Midbrain Dopamine Neurons

Co-culture and Interaction Studies

A Co-culture Technique for Differentiating Human Neural Progenitor Cells into Neurons

Co-culturing Glutamatergic Neurons and Pediatric High-Grade Glioma Cells

An Indirect Neuron-Astrocyte Co-Culture Technique for Studying Neuron-Glia Interactions

Developing a Neuron and Macrophage Coculture In Vitro

Using a Macrophage Conditioned Medium to Promote Neuron Extensions

Co-culturing of Ventral Hippocampal and Nucleus Accumbens

Establishing a Co-Culture of Dental Pulp Cells and Trigeminal Neurons for Cross-Communication

Establishing a Co-Culture of Interneurons with Periventricular Endothelial Cells

Establishing a Brain Tumor in an Organotypic Slice Co-culture Model

Studying the Regeneration of Functional Connections between Spinal Cord Slices Using a Multi-Electrode Array

Isolating Tumor-Infiltrating Immune Cells from a Murine Brain

Selectively Removing Microglia from a Rat Cerebellar Granule Neuron Culture

Generating 3D Co-culture Spheres of Astrocytes and Neurons to Induce Synapse Formation

Isolation of DRG Neurons and Coculture with Schwann Cell Precursors to Generate Schwann Cells

Developing a Triple Cell Culture Model of the Human Blood-Brain Barrier

A Co-culture Method to Model Neuron-Oligodendrocyte Interactions

Analyzing Tooth Germ and Trigeminal Ganglia Interactions Using a Microfluidic Co-culture System

Coculturing of Retinal Ganglion Neurons with Olfactory Ensheathing Glia

Neuronal Enrichment of Dorsal Root Ganglia by Immunopanning

Differentiating Human Neural Stem Cells into Neural and Astrocyte Progenitors

Analyzing Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants

Generating Forebrain-Type Cerebral Organoids from Human-Induced Pluripotent Stem Cells

Labeling Neural Cell Surface Markers with Azidosugar while Co-culturing with Endothelial Cells

Co-Culture of Schwann Cells with DRG Neurons on a Pre-Stretched Membrane

Co-culturing of a Dorsal Root Ganglion Explant with Schwann Cells for Neuron Myelination

Isolating Sensory Neurons and Co-Culturing with Natural Killer Cells

Employing Reporter Cells in a Co-Culture to Study Retinoic Acid Production by Mouse Embryonic Cells

Generating a 3D Co-Culture Model of Human Corneal Fibroblasts and Neurons

Advanced Neuronal Models

Generating Neural Progenitors from Embryonic Stem Cells in Serum-Free Monolayer Cultures

Differentiating Human Neuronal Stem Cells into 3D Culture Using a Porous Scaffold

Generating Postganglionic Sympathetic Neurons from Human Pluripotent Stem Cells

Generation and Differentiation of Primary Neurospheres from Zebrafish Neural Stem Cells

An In Vitro Method to Generate Human Brain Organoids

Electrical Stimulation-Induced Differentiation of Neural Stem and Progenitor Cells

Generating Induced Pluripotent Stem Cell-Derived Cerebral Organoids

Generating Neuromuscular Junctions Using a Microfluidic Device

Building Three-Dimensional Neuronal Networks Coupled to Micro-Electrode Arrays

Developing Neuron Balls Using a Hanging Drop Culture Technique

Tracking Axonal Transport of Organelles in Motor Neurons Using a Microfluidic Device

Direct Neuronal Reprogramming of Mouse Astrocytes into Neurons

Preparing Wedge Brain Slices to Visualize Auditory Neurons

Stimulating Neuronal Cell Differentiation Using Gold Nanorods

Growth and Differentiation of Magnetic Nano Particle-Loaded Neurons on Magnetic Platforms

Differentiating Human Embryonic Stem Cells into Neural Progenitors

Preparing Free-Floating Slice Cultures from an Adult Human Brain

Preparation of Organotypic Slice Cultures from a Rat Pup's Hippocampus

Preparation of Brain Sections Using a NMDG-Based Protective Recovery Method

A Technique for the Prolonged Incubation of Neuronal Tissue of the Retina

Generating Mature Cerebellar Organoids From Induced Pluripotent Stem Cells Using Single-Use Bioreactors

Establishing a Three-Dimensional Culture of Rat Brain-Derived Glial Cells

Generating and Maintaining Astrospheres Using Human Pluripotent Stem Cells

Differentiating Non-Transgenic and Transgenic Human Pluripotent Stem Cells into Neural Progenitor Cells

Generating Schwann Cell-Like Cells from Bone Marrow Stromal Cells

An In Vitro Method to Generate Astrocyte Scaffolds within Hydrogel Micro-columns

Developing an Engineered Silk-Collagen-Based 3D Model of Polarized Neural Tissue

Replating Differentiated Neurons Derived from Human Pluripotent Stem Cells

Developing Human iPSC-Derived Motor Nerve Organoids Using a Microfluidic Chip

Stimulating Nerve Stem Cell Differentiation Using Cold Atmospheric Plasma Treatment

Generating Cortical Interneuron Precursors From Mouse Embryonic Stem Cells

Direct Reprogramming of Hematopoietic Progenitor Cells into Neural Stem Cells

siRNA-Mediated Gene Silencing in Neural Stem Cells

Differentiating Induced Neural Progenitor Cells into Astrocytes

A Technique for Generating Neural Progenitor Cells from Mouse Embryonic Stem Cells

Differentiation of Neural Progenitor Cells into Neurons

A Modified Roller Tube Method for the Long-Term Culture of Rodent Brain Slices

Initiating Rapid Neurite Extension and Formation Functional Neuronal Connections

Generating Neurons by Reprogramming Human Dermal Fibroblast Cells

Generating and Maintaining Slice Cultures from Human Glioblastoma Tissue

Isolation and Culture of Primary Schwann Cells from a Human Dermis

A Method for 3D Bioprinting Murine Cortical Astrocytes

A Hyaluronic Acid-Based Hydrogel for 3-Dimensional Culture of Glioblastoma Cells

Analyzing Purkinje Cell Survival in Organotypic Cerebellar Slice Cultures

Generating an Organoid Culture from a Brain Tumor Cell Suspension

Differentiating Human Embryonic Stem Cells into Oligodendrocytes

A Technique to Generate a 2D Monolayer of Cerebellar Cells from Induced Pluripotent Stem Cells

Generating Neurons and Glial Cells from Human Induced Pluripotent Stem Cell-Derived Embryoid Bodies

Isolating and Culturing Cells from Diffuse Intrinsic Pontine Glioma

Differentiation of Spinal Cord Neural Progenitor Cells into Motor Neurons

Generation of Spheroids from Human Pluripotent Stem Cells

In Vitro Induction of Neuromuscular Junctions from Engineered Human-Induced Pluripotent Stem Cells

Differentiation of Neural Stem Cells to Neurons Using a Compartmentalized Microfluidic Device

Enhancing Stem Cell Differentiation with a Transient DMSO Treatment

Isolation of Stem Cells from Postnatal Mouse Cerebellum and Differentiation into Neural Cells

Isolating and Culturing Mouse Cerebral Pericytes

A Technique to Isolate Satellite Cells From Rat Head Muscle Tissue

A Technique to Optimize Cerebral Organoid Culture Using Lateral Soft Light Illumination

Retinoic Acid-Induced Neurogenesis using Mouse Embryonic Carcinoma Cells

Preparation of a Micropatterned Substrate to Study Schwann Cell Phenotypes

Differentiating Embryoid Body Cells into Neural Progenitors Using Retinoic Acid

Preparing Cerebral Endothelial Tubes from Mouse Parenchymal Arterioles

An In Vitro Transduction-Induced Myelination Assay for Oligodendrocyte Precursor Cells

Generating a Pro-Inflammatory Organ Culture Model to Simulate an Early Intervertebral Disc Disease

A Method for Generating Oligodendrocyte-Conditioned Medium

Human Pluripotent Stem Cell Differentiation in Neurons Using Engineered Lentiviruses

Isolating Cranial Neural Folds From a Chick Embryo for a Neural Crest Cell Culture

Differentiating Mouse Embryonic Stem Cells into a Networked Neuron Population

A Technique to Generate Brain Organoids from Human Embryonic Stem Cells

Magnetic Separation of Schwann Cells from Mouse Sciatic Nerves

Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from a Mouse Embryo

A Technique to Isolate Nuclei from Non-neuronal Cells in the Hippocampal Dentate Gyrus

Handling Neuronal Stem Cell Cultures in a Cell Production Facility

Cell Fate Reprogramming of Nematode Germ Cells into Neurons

Purification of CD31⁺ Endothelial Precursor Cells Using Magnetic-Activated Cell Sorting

Differentiating Human-Induced Pluripotent Stem Cells into Microglia-Like Cells

Formation and Expansion of Neurospheres from a Hippocampal Tissue

Establishing a Müller Glial Cell Culture Obtained from Mouse Retinas

Generating Brain Endothelial Cells from Induced Pluripotent Stem Cells

Establishing Primary Mixed Glial Cell Cultures from a Mouse Spinal Cord

Isolating Adipose-Derived Stem Cells from Murine Periaortic Adipose Tissue

Differentiating Otic Progenitor Cells Into Sensory Epithelial Cells Followed by Immunostaining

Isolating Enteric Glial Cells From the Submucosa and Lamina Propria of a Mouse

Differentiating Immortalized Multipotent Otic Progenitors into Spiral Ganglion Neurons and Evaluating the Differentiation

Purifying Capillaries From a Porcine Brain

Ex Vivo Culture and Imaging of Oculomotor Slices from Transgenic Mouse Embryos

A Method for the Isolation of Neurovascular Units from Rat Brain

Differentiating Human-Induced Pluripotent Stem Cells into Brain Microvascular Endothelial Cells

Generating Cortical Pyramidal Neurons from Human Neural Stem Cells

Isolating and Culturing Chick Embryonic Neurons on a Multi-Electrode Array

Generating a Blood-Brain Barrier Chip Using iPSC-derived Precursor Cells

Generation of Medial Thalamus-Anterior Cingulate Cortex Slices from Mouse Brains

A Lentiviral Transcriptional Reporter System to Generate a Glioblastoma Stem Cell Differentiation Reporter Line

Infecting Primary Mouse Midbrain Neurons with Engineered Adeno-Associated Viruses

Preparing Rhinal Cortex-Hippocampus Slices from a Rat Pup Brain

Dissection and Mounting of Fruit Fly Larval Brain Explants

Differentiation of Induced Pluripotent Stem Cells into Neural Progenitor Cells

Development and Maintenance of Serum-Free Embryoid Bodies

Constructing Patterned Neuronal Circuits on a Multi-Electrode Array

Differentiating Engineered Human Induced Pluripotent Stem Cells into Functional Motor Neurons