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Screening für Amyloid-Aggregation durch Semi-denaturierenden Detergens-Agarose-Gel-Elektrophorese
JoVE Journal
Biologie
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JoVE Journal Biologie
Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis
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Screening für Amyloid-Aggregation durch Semi-denaturierenden Detergens-Agarose-Gel-Elektrophorese

26,263 Views

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10:03 min

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July 16, 2008

DOI:

10.3791/838-v

DOI:
10.3791/838-v

•

10:03 min
July 16, 2008

•

10 Views
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1Whitehead Institute for Biomedical Research, 2Department of Biology,MIT – Massachusetts Institute of Technology, 3Howard Hughes Medical Institute

Summary

Automatically generated

SDD-AGE ist eine nützliche Technik für die Erkennung und Charakterisierung von Amyloid-ähnlichen Polymeren in den Zellen. Hier zeigen wir eine Anpassung, dass diese Technik zugänglich großflächige Anwendungen macht.

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Amyloid AggregationProtein MisfoldingPrionsConformationally Self-templating ProteinsLower OrganismsInsolubilityStructural HeterogeneitySemi-Denaturing Detergent-Agarose Gel Electrophoresis (SDD-AGE)Size-based ResolutionDetergent InsolubilityAmyloid PolymersDetectionCharacterizationConformational VariantsLarge-scale ApplicationsScreens For Prions And Amyloidogenic ProteinsSDD-AGE MethodCapillary TransferReliabilityEase Of UseSDS-insoluble Conformers

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Cite this Article

Halfmann, R., Lindquist, S. Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis. J. Vis. Exp. (17), e838, doi:10.3791/838 (2008).

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