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Entwicklungsbiologie

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Single Cell and Nuclei Extraction from Human Brain Organoids

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Generation and Downstream Analysis of single-cell and single-nuclei transcriptomes in brain organoids(뇌 오가노이드에서 단세포 및 단핵체 전사체의 생성 및 다운스트림 분석)

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Entwicklungsbiologie

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JoVE Journal Entwicklungsbiologie

Generation and Downstream Analysis of Single-Cell and Single-Nuclei Transcriptomes in Brain Organoids

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Generation and Downstream Analysis of single-cell and single-nuclei transcriptomes in brain organoids(뇌 오가노이드에서 단세포 및 단핵체 전사체의 생성 및 다운스트림 분석)

Single Cell and Nuclei Extraction from Human Brain Organoids

DOI:

10.3791/66225-v

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02:19 min

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March 29, 2024

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Miriam Wandres*^1,2, Denise Aigner*^1,2, Nicolai Kastelic³, Anastasiya Boltengagen, Agnieszka Rybak-Wolf³, Nikolaus Rajewsky²

¹Berlin Institute for Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine (MDC), ²Institut für Biologie,Humboldt-Universität zu Berlin, ³Organoid platform, Berlin Institute for Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine (MDC)

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Single-cell Transcriptomics Single-nucleus Transcriptomics Brain Organoids Cell Type Identification Gene Expression Cell Lineage Developmental Processes Molecular Mechanisms

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Generation and Downstream Analysis of single-cell and single-nuclei transcriptomes in brain organoids(뇌 오가노이드에서 단세포 및 단핵체 전사체의 생성 및 다운스트림 분석)

Isolation of single cells from human brain organoids(단일 세포 RNA 염기서열 분석)를 위한 인간 뇌 오가노이드에서 단일 세포 분리

Single-Nucleus RNA Sequencing을 위한 인간 뇌 오가노이드에서 단일 핵 분리

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