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Journal
/
Biochemie
/
Strategies for Expressing and Purifying Solute Carrier Transporters
/
Author Spotlight: Expression and Purification of Human Solute Carrier Transporters Using Codon-Optimized Genes
JoVE Journal
Biochemie
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JoVE Journal
Biochemie
High-Throughput Expression and Purification of Human Solute Carriers for Structural and Biochemical Studies
Author Spotlight: Expression and Purification of Human Solute Carrier Transporters Using Codon-Optimized Genes
Strategies for Expressing and Purifying Solute Carrier Transporters
DOI:
10.3791/65878-v
•
02:10 min
•
September 29, 2023
•
Sagar Raturi
,
Huanyu Li
,
Yung-Ning Chang
,
Andreea Scacioc
,
Tina Bohstedt
,
Alejandra Fernandez-Cid
,
Adam Evans
,
Patrizia Abrusci
,
Abilasha Balakrishnan
,
Tomas C. Pascoa
,
Didi He
,
Gamma Chi
,
Nanki Kaur Singh
,
Mingda Ye
,
Anna Li
,
Leela Shrestha
,
Dong Wang
,
Eleanor P. Williams
,
Nicola A. Burgess-Brown
,
Katharina L. Dürr
,
Vera Puetter
,
Alvaro Ingles-Prieto
,
David B. Sauer
1
Centre for Medicines Discovery, Nuffield Department of Medicine
,
University of Oxford
,
2
Nuvisan ICB GmbH
,
3
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences
Tags
Solute Carriers
SLCs
Membrane Transporters
Drug Discovery
High-throughput Expression
Protein Purification
Structural Studies
Biochemical Studies
ReSOLUTE
BacMam Expression System
Cryo-EM
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Author Spotlight: Expression and Purification of Human Solute Carrier Transporters Using Codon-Optimized Genes
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