/

Entwicklungsbiologie

/

Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

/

Isolamento Intestinal de Larvas de Zebrafish para Sequenciamento de RNA de Célula Única

JoVE Journal
Entwicklungsbiologie
Author Produced

Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Entwicklungsbiologie

Gut Isolation from Zebrafish Larvae for Single-cell RNA Sequencing

Isolamento Intestinal de Larvas de Zebrafish para Sequenciamento de RNA de Célula Única

Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

DOI:

10.3791/65876-v

•

01:24 min

•

November 10, 2023

•

Naomi J. M. Kakiailatu, Laura E. Kuil², Eric Bindels, Joke T. M. Zink, Michael Vermeulen, Veerle Melotte, Maria M. Alves

¹Department of Clinical Genetics,Erasmus University Medical Center – Sophia Children’s Hospital, ²Division of Psychosocial Research and Epidemiology,the Netherlands Cancer Institute, ³Department of Hematology,Erasmus Medical Center, ⁴Department of Pathology, GROW-School for Oncology and Developmental Biology,Maastricht University Medical Center

Tags

Zebrafish Gut Isolation Single-cell RNA Sequencing Gastrointestinal Development Enteric Nervous System Intestinal Cell Types Epithelial Cells Stromal Cells Blood Cells Muscle Cells Immune Cells Enteric Neurons Enteric Glia ScRNA-seq Congenital Defects Enteric Neuromuscular Disorders Gastrointestinal Health And Disease

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Isolamento Intestinal de Larvas de Zebrafish para Sequenciamento de RNA de Célula Única

Obtenção de larvas de peixe-zebra selvagem e transgênico e dissociação de larvas inteiras de tipo selvagem

Isolamento e Dissociação do Intestino de Larvas de Zebrafish para Enriquecimento de Células Intestinais FACS

Enriquecimento FACS de Suspensão de Células Intestinais de Zebrafish para scRNAseq

Read Article