Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Normothermic Ex Vivo Liver Machine Perfusion in Mouse
Kapitel
- 00:09Introduction
- 00:40Preparation
- 04:33Liver Explanation
- 09:49Liver and Chamber Connection
- 10:53Adjust the Flow Rate According to PV Pressure
- 11:19Representative Results
- 12:23Conclusion
A normothermic ex vivo liver perfusion (NEVLP) system was created for mouse livers. This system requires experience in microsurgery but allows for reproducible perfusion results. The ability to utilize mouse livers facilitates the investigation of molecular pathways to identify novel perfusate additives and enables the execution of experiments focused on organ repair.
Tags
NormothermicEx-vivoLiverMachine PerfusionMouseMolecular MechanismsGenetically Modified AnimalsTransgenic MiceThermostatic ControllerPeristaltic PumpOxygenatorBubble TrapPortal VeinSuprahepatic CavaBile DrainageSemi-sterile30G Needle26G CatheterHeparinized SalineOrgan ChamberRoller PumpHeat Exchanger
