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İnsan kaynaklı pluripotent kök hücre kaynaklı kardiyomiyositlerde kalsiyum ve kontraktilite parametrelerinin gerçek zamanlı ölçümleri

JoVE Journal
Biologie

This content is Free Access.

JoVE Journal Biologie

Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

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İnsan kaynaklı pluripotent kök hücre kaynaklı kardiyomiyositlerde kalsiyum ve kontraktilite parametrelerinin gerçek zamanlı ölçümleri

1,292 Views

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01:21 min

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May 26, 2023

DOI:

10.3791/65326-v

DOI:

10.3791/65326-v

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01:21 min

May 26, 2023

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5 Views

Rafeeh Dinani², Emmy Manders^2,3, Michiel Helmes^2,3, Lili Wang, Bjorn C. Knollmann, Diederik W. D. Kuster², Jolanda van der Velden²

¹Division of Physiology, Amsterdam UMC,Vrije University, ²Heart Failure & Arrhythmias,Amsterdam Cardiovascular Sciences, ³CytoCypher BV, ⁴Division of Clinical Pharmacology,Vanderbilt School of Medicine

Tags

Human Induced Pluripotent Stem Cell-derived Cardiomyocytes HiPSC-CMs Contractility Calcium Handling Real-time Measurements Pixel Correlation Calcium-sensitive Fluorophore Fura-2 2D Platform Preclinical Drug Screening

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