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Real-Time Measurements of Calcium and Contractility Parameters in hiPSC-CMs
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实时测量人诱导多能干细胞来源心肌细胞中的钙和收缩力参数
JoVE Journal
Biologie
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JoVE Journal Biologie
Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
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实时测量人诱导多能干细胞来源心肌细胞中的钙和收缩力参数

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01:21 min

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May 26, 2023

DOI:

10.3791/65326-v

DOI:
10.3791/65326-v

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01:21 min
May 26, 2023

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5 Views
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1Division of Physiology, Amsterdam UMC,Vrije University, 2Heart Failure & Arrhythmias,Amsterdam Cardiovascular Sciences, 3CytoCypher BV, 4Division of Clinical Pharmacology,Vanderbilt School of Medicine

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Human Induced Pluripotent Stem Cell-derived CardiomyocytesHiPSC-CMsContractilityCalcium HandlingReal-time MeasurementsPixel CorrelationCalcium-sensitive FluorophoreFura-22D PlatformPreclinical Drug Screening

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Cite this Article

Dinani, R., Manders, E., Helmes, M., Wang, L., Knollmann, B. C., Kuster, D. W. D., van der Velden, J. Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. J. Vis. Exp. (195), e65326, doi:10.3791/65326 (2023).

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