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Journal
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Biochemie
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Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities
/
Memelilerde Mitokondriyal Fonksiyonu Ölçmek için Oksijenden Bağımsız Tahliller
JoVE Journal
Biochemie
This content is Free Access.
JoVE Journal
Biochemie
Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
Please note that all translations are automatically generated.
Click here for the English version.
Memelilerde Mitokondriyal Fonksiyonu Ölçmek için Oksijenden Bağımsız Tahliller
Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities
DOI:
10.3791/65184-v
•
01:14 min
•
May 19, 2023
•
Tenzin Tseyang*
1
,
Jonathan Valeros*
1
,
Paula Vo*
1
,
Jessica B. Spinelli
2
1
Program in Molecular Medicine
,
University of Massachusetts Chan Medical School
,
2
Cancer Center
,
University of Massachusetts Chan Medical School
Tags
Mitochondrial Function
Oxygen-independent Assays
Hypoxia
Metabolite Analysis
Fumarate
Electron Transport Chain
ATP Production
Pyrimidine Biosynthesis
NADH Oxidation
Superoxide Production
Respirometry
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