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Journal
/
Biochemie
/
Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities
/
Author Spotlight: Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
JoVE Journal
Biochemie
This content is Free Access.
JoVE Journal
Biochemie
Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
Author Spotlight: Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities
DOI:
10.3791/65184-v
•
01:14 min
•
May 19, 2023
•
Tenzin Tseyang*
1
,
Jonathan Valeros*
1
,
Paula Vo*
1
,
Jessica B. Spinelli
2
1
Program in Molecular Medicine
,
University of Massachusetts Chan Medical School
,
2
Cancer Center
,
University of Massachusetts Chan Medical School
Tags
Mitochondrial Function
Oxygen-independent Assays
Hypoxia
Metabolite Analysis
Fumarate
Electron Transport Chain
ATP Production
Pyrimidine Biosynthesis
NADH Oxidation
Superoxide Production
Respirometry
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Author Spotlight: Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement
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