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In Vitro Investigation of the Effects of the Hyaluronan-Rich Extracellular Matrix on Neural Crest Cell Migration
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
In Vitro Investigation of the Effects of the Hyaluronan-Rich Extracellular Matrix on Neural Crest Cell Migration

In Vitro Investigation of the Effects of the Hyaluronan-Rich Extracellular Matrix on Neural Crest Cell Migration

DOI:
10.3791/64749-v

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11:16 min

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February 10, 2023

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1Department of Orthodontics and Dentofacial Orthopedics,Osaka University Graduate School of Dentistry

Kapitel

  • 00:04Introduction
  • 01:04Culture of Mouse Cranial Neural Crest Cells
  • 03:30Preparation of the HA/Col1-Coated Dish
  • 04:49Migration Assay on the HA/Col1-Coated Dish
  • 06:44Results: TMEM2 Expression in NCCs and HA Degradation
  • 09:10Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol outlines an in vitro migration experiment suitable for the functional analysis of the molecules involved in the in vivo migration of neural crest cells into the hyaluronan-rich extracellular matrix.

Tags

Hyaluronic AcidExtracellular MatrixNeural Crest Cell MigrationIn Vitro ModelBasement Membrane MatrixO9-1 CellsCell CultureCell MigrationCell AdhesionDrug ScreeningHA Degradation

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