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Immunology and Infection

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JoVE Journal Immunology and Infection
Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
 

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Article DOI: 10.3791/64288-v • 10:16 min • August 17th, 2022
August 17th, 2022 •
*1, *1, 1, 1, 1, 1, 1, 2, 1, 3, 1
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, Third Military Medical University, 2Department of Pediatrics, Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital, Third Military Medical University
* These authors contributed equally

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Summary
Automatic Translation

A self-assembled peptide-poloxamine nanoparticle (PP-sNp) is developed using a microfluidic mixing device to encapsulate and deliver in vitro transcribed messenger RNA. The described mRNA/PP-sNp could efficiently transfect cultured cells in vitro.

Tags

Peptide-poloxamine Nanoparticles MRNA Delivery System Efficient Transfection In Vitro Transcribed MRNA Cultured Cells Mucosal MRNA Vaccine Low-cost Materials Short Protocol Bronchial Epithelial Cells Mucosal COVID-19 Vaccines Lung Cancers Cystic Fibrosis Gene Therapy Metridia Luciferase PUC57 Vector Linearized DNA Template T7 Transcription Kit Pseudouridine Uncapped IVT MRNA
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