Journal
/
Neurowissenschaften
/
Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes
JoVE Journal
Neurowissenschaften
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Neurowissenschaften
Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes

Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes

DOI:
10.3791/64175-v

•

07:25 min

•

July 07, 2022

•

, ,
1Institute of Stem Cell Research, Helmholtz Zentrum München,German Research Center for Environmental Health, 2Department of Physiological Genomics, Biomedical Center Munich,Ludwig-Maximilians University, 3Graduate School of Systemic Neurosciences, BioCenter,Ludwig-Maximilians University

Kapitel

  • 00:04Introduction
  • 00:55Spinal Cord Tissue Dissection
  • 01:34Magnetic Activated Cell Sorting (MACS)
  • 02:39Seeding of Astrocytes for Reprogramming
  • 04:25Reprogramming of Astrocytes (7‐Day Analysis)
  • 05:29Results: Murine Astrocyte Cultures to Study Direct Neuronal Reprogramming
  • 06:37Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here we describe a detailed protocol to generate highly enriched cultures of astrocytes derived from different regions of the central nervous system of postnatal mice and their direct conversion into functional neurons by the forced expression of transcription factors.

Tags

AstrocytesNeuronal ReprogrammingCell IsolationSpinal CordCell CultureAstrocyte CulturePoly-D-LysineEGFBFGF

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Isolation and Culture of Mouse Cortical Astrocytes

Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction

Sex Differences in Mouse Hippocampal Astrocytes after In-Vitro Ischemia

Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

Chronic Transcranial Electrical Stimulation and Intracortical Recording in Rats

Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors

In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes

Neuronal Differentiation from Mouse Embryonic Stem Cells In vitro

In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

Read Article