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通过
清除技术对稻田进行深度荧光观察
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal
Biologie
Deep Fluorescence Observation in Rice Shoots
via
Clearing Technology
Please note that all translations are automatically generated.
Click here for the English version.
通过
清除技术对稻田进行深度荧光观察
DOI:
10.3791/64116-v
•
07:21 min
•
June 27, 2022
•
Yoko Niimi
,
Keisuke Nagai
,
Motoyuki Ashikari
,
Yoko Mizuta
4
1
Graduate School of Bioagricultural Sciences
,
Nagoya University
,
2
Bioscience and Biotechnology Center
,
Nagoya University
,
3
Institute for Advanced Research (IAR)
,
Nagoya University
,
4
Institute of Transformative Bio-Molecules (ITbM)
,
Nagoya University
Kapitel
00:04
Introduction
00:53
Sampling
01:46
Fixation of the Samples
02:53
Trimming the Fixed Sample
03:47
Treatment with the Clearing Solution
04:46
Results: Observation of the Depth of Fluorescence
06:32
Conclusion
Summary
Automatische Übersetzung
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Automatische Übersetzung
本方案描述了一种用于稻芽的清除技术，由于组织的坚硬，厚厚或分层的性质，这些苗很难为内部结构观察做准备。这种方法有助于连续和深入的荧光观察，即使在成年水稻植物中也是如此。
Tags
Keywords: Deep Fluorescence Observation
Rice Shoots
Clearing Technology
Confocal Microscope
Macro Perspective
Adult-erized Shoots
Wide Plant Species
Plant Biological Research
Shoot Apical Meristem
Young Panicle
Oval Cross Section
Fixative Solution
Paraffin Film
Desiccator
Vacuum Pump
Micro-slicer Tray
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