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Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

DOI:
10.3791/64111-v

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08:26 min

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August 23, 2022

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, , , ,
1Department of Cellular Neurobiology, Graduate School of Medicine and Faculty of Medicine,The University of Tokyo

Kapitel

  • 00:04Introduction
  • 01:00AAV Injection
  • 03:04Cranial Window Implantation
  • 04:36In Vivo Two‐Photon Imaging of Microglia and Neuronal Calcium Dynamics
  • 06:42Results: Microglial Dynamics and Visual Responses in a Dendritic Spine
  • 07:44Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here, we describe a protocol combining adeno-associated virus injection with cranial window implantation for simultaneous imaging of microglial dynamics and neuronal activity in awake mice.

Tags

Simultaneous ImagingMicroglia DynamicsNeuronal ActivityAwake MiceAAV InjectionCranial WindowStereotaxic InstrumentMotion ArtifactPrimary Visual CortexMicroinjector

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