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Journal / Cancer Research / Rapid Optical Clearing for Semi-High-Throughput…
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Cancer Research

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JoVE Journal Cancer Research
Rapid Optical Clearing for Semi-High-Throughput Analysis of Tumor Spheroids
 

Rapid Optical Clearing for Semi-High-Throughput Analysis of Tumor Spheroids

Article DOI: 10.3791/64103-v • 08:12 min • August 23rd, 2022
August 23rd, 2022 •
1, 2, 1
1The University of Queensland Diamantina Institute, The University of Queensland, 2School of Mathematical Sciences, Queensland University of Technology

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Summary
Automatic Translation

Tumor spheroids are becoming increasingly utilized to assess tumor cell-microenvironment interactions and therapy response. The present protocol describes a robust but simple method for semi-high-throughput imaging of 3D tumor spheroids using rapid optical clearing.

Tags

Rapid Optical Clearing Semi-High-Throughput Analysis Tumor Spheroids Tumor Modeling Data Collection Protocol Chemicals Equipment Agarose PBS N,N,N'N'Tetrakis(2-hydroxypropyl)ethylenediamine Urea Sucrose Triton X-100 Deionized Water Spheroid Generation Agarose Overlay Method Pipette
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