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FACS-isolering og dyrkning af fibro-adipogene forfædre og muskelstamceller fra uforstyrret og skadet museskeletmuskel
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1Laboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS),National Institutes of Health (NIH), 2Flow Cytometry Section, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS),National Institutes of Health (NIH)
Kapitel
- 00:04Introduction
- 01:04Hind Limb Muscle Harvesting
- 03:11Mechanical and Enzymatic Muscle Digestion
- 05:04Generation of Mononucleated Cells
- 07:03Antibody Staining for Flow Cytometry
- 08:49Results: FACS-Assisted Simultaneous Isolation and Culture of FAPs and MuSCs from Mouse Limb Muscles
- 10:37Conclusion
Den præcise identifikation af fibro-adipogene stamceller (FAP'er) og muskelstamceller (MuSC'er) er afgørende for at studere deres biologiske funktion under fysiologiske og patologiske forhold. Denne protokol giver retningslinjer for isolering, rensning og kultur af FAP'er og MuSC'er fra voksne musemuskler.
