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FACS-عزل وثقافة السلف الليفي الأديبوجينيك والخلايا الجذعية العضلية من العضلات الهيكلية للفئران غير المضطربة والمصابة
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1Laboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS),National Institutes of Health (NIH), 2Flow Cytometry Section, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS),National Institutes of Health (NIH)
Kapitel
- 00:04Introduction
- 01:04Hind Limb Muscle Harvesting
- 03:11Mechanical and Enzymatic Muscle Digestion
- 05:04Generation of Mononucleated Cells
- 07:03Antibody Staining for Flow Cytometry
- 08:49Results: FACS-Assisted Simultaneous Isolation and Culture of FAPs and MuSCs from Mouse Limb Muscles
- 10:37Conclusion
