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エチレンジアミン四酢酸媒介接着消によるフィーダー細胞上のヒト多能性幹細胞の迅速で費用対効果の高い酵素フリー継代

JoVE Journal
Biologie
Author Produced

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JoVE Journal Biologie

Rapid, Cost-Efficient, Enzyme-Free Passaging of Human Pluripotent Stem Cells on Feeder Cells by Ethylenediaminetetraacetic Acid-Mediated Dis-Adhesion

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エチレンジアミン四酢酸媒介接着消によるフィーダー細胞上のヒト多能性幹細胞の迅速で費用対効果の高い酵素フリー継代

203 Views

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07:21 min

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July 07, 2023

DOI:

10.3791/63788-v

DOI:

10.3791/63788-v

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07:21 min

July 07, 2023

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1 Views

Hege Brincker Fjerdingstad, Joel C. Glover²

¹Norwegian Center for Stem Cell Research, Department of Immunology and Transfusion Medicine,Oslo University Hospital, ²Laboratory of Neural Development and Optical Recording (NDEVOR), Department of Molecular Medicine, Institute of Basic Medical Sciences,University of Oslo

Tags

Human Pluripotent Stem Cells Feeder Cells Enzyme-free Passaging EDTA-mediated Dis-adhesion Rapid Cost-efficient Mitotic Arrest Gamma Irradiation

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