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एथिलीनडायमिनेटेट्राएसेटिक एसिड-मध्यस्थता डिस-आसंजन द्वारा फीडर कोशिकाओं पर मानव प्लुरिपोटेंट स्टेम कोशिकाओं का तेजी से, लागत कुशल, एंजाइम-मुक्त पासिंग
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JoVE Journal Biologie
Rapid, Cost-Efficient, Enzyme-Free Passaging of Human Pluripotent Stem Cells on Feeder Cells by Ethylenediaminetetraacetic Acid-Mediated Dis-Adhesion
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एथिलीनडायमिनेटेट्राएसेटिक एसिड-मध्यस्थता डिस-आसंजन द्वारा फीडर कोशिकाओं पर मानव प्लुरिपोटेंट स्टेम कोशिकाओं का तेजी से, लागत कुशल, एंजाइम-मुक्त पासिंग

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07:21 min

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July 07, 2023

DOI:

10.3791/63788-v

DOI:
10.3791/63788-v

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07:21 min
July 07, 2023

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1 Views
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1Norwegian Center for Stem Cell Research, Department of Immunology and Transfusion Medicine,Oslo University Hospital, 2Laboratory of Neural Development and Optical Recording (NDEVOR), Department of Molecular Medicine, Institute of Basic Medical Sciences,University of Oslo

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Human Pluripotent Stem CellsFeeder CellsEnzyme-free PassagingEDTA-mediated Dis-adhesionRapidCost-efficientMitotic ArrestGamma Irradiation

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Fjerdingstad, H. B., Glover, J. C. Rapid, Cost-Efficient, Enzyme-Free Passaging of Human Pluripotent Stem Cells on Feeder Cells by Ethylenediaminetetraacetic Acid-Mediated Dis-Adhesion. J. Vis. Exp. (197), e63788, doi:10.3791/63788 (2023).

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