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Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
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JoVE Journal Biochemie
Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

2,176 Views

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09:47 min

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May 10, 2022

DOI:

10.3791/63561-v

DOI:
10.3791/63561-v

•

09:47 min
May 10, 2022

•

4 Views
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1Department of Genetic and Evolution,Federal University of São Carlos, 2Department of Biochemistry and Immunology, Faculty of Medicine of Ribeirão Preto,University of São Paulo

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Keyword Extraction:Substrate UbiquitylationE3 LigaseMammalian Cell LysatesImmunoprecipitation AssayWestern BlotHuman DiseasesCancerNeurological DisordersE3 Ligase DysregulationHEK293T Cell LineDulbecco’s Modified Eagles MediumFetal Bovine SerumPenicillinStreptomycinL-glutamineTrypsin EDTAPolyethyleneimineOpti-MEM 1 Reduced Serum MediumTransient Transfections

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dos Passos, P. M. S., Spagnol, V., de Correia, C. R., Teixeira, F. R. Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates. J. Vis. Exp. (183), e63561, doi:10.3791/63561 (2022).

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