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A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage
1Oxidative Stress Group, Dept. Cell Biology, Microbiology and Molecular Biology, College of Arts and Sciences,University of South Florida, 2Cepheid (Danaher Corp.),US Technical Operations, 3Department of Clinical Laboratory Sciences, College of Applied Medical Sciences,King Saud University, 4Department of Chemistry and Biochemistry, Institute of Environment,Florida International University, 5Leicester School of Allied Health Sciences, Faculty of Health and Life Sciences,De Montfort University
Kapitel
- 00:05Introduction
- 01:10Cell Lysis
- 02:56Electrophoresis
- 04:42Propidium Iodide Staining
- 05:43Results: Analyzing DNA Damage Using High Throughput Alkaline Comet Assay
- 07:17Conclusion
The comet assay is a popular means of detecting DNA damage. This study describes an approach to running slides in representative variants of the comet assay. This approach significantly increased the number of samples while decreasing assay run-time, the number of slide manipulations, and the risk of damage to gels.
