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Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
1Centre for Women, Children, and Reproduction,Guangxi Key Laboratory of Metabolic Diseases Research, 2Department of Biopharmaceutics, School of Laboratory Medicine and Biotechnology,Southern Medical University, 3Department of Obstetrics and Gynecology,Chinese PLA General Hospital, 4Clinical Laboratory,Northern Theater Air Force Hospital, 5Guangzhou Darui Reproduction Technology Co., Ltd.
Kapitel
- 00:04Introduction
- 00:40Fragment Selection Procedure
- 02:20Sequencing Template Preparation and Enrichment
- 05:48Loading the Sample and Sequencing
- 07:22Results: Whole Genome Amplification Combined with Next-Generation Sequencing for Analysis of Embryo Aneuploidy
- 08:59Conclusion
The protocol presents the overall in-lab procedures required in pre-implantation genetic testing for aneuploidy on a semiconductor-based next-generation sequencing platform. Here we present the detailed steps of whole genome amplification, DNA fragment selection, library construction, template preparation, and sequencing working flow with representative results.
