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Optical Clearing of Plant Tissues for Fluorescence Imaging
JoVE Journal
Biologie
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JoVE Journal Biologie
Optical Clearing of Plant Tissues for Fluorescence Imaging

Optical Clearing of Plant Tissues for Fluorescence Imaging

DOI:
10.3791/63428-v

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04:55 min

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January 05, 2022

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, , , ,
1Institute of Transformative Bio-Molecules (ITbM),Nagoya University, 2Institute for Advanced Research (IAR),Nagoya University, 3Division of Biological Science, Graduate School of Science,Nagoya University, 4Department of Biological Sciences, Graduate School of Science,The University of Tokyo

Kapitel

  • 00:05Introduction
  • 00:48Fixation of Samples
  • 01:48Clearing
  • 02:21Observation
  • 02:59Results: Optical Clearing and Fluorescence Imaging of Tissues
  • 04:18Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here, a method is described for making plant tissues transparent while maintaining the stability of fluorescent proteins. This technique facilitates deep imaging of cleared plant tissues without physical sectioning.

Tags

Optical ClearingFluorescence ImagingWhole-plant ImagingPlant-microbe InteractionsFixationFluorescent ProteinVacuum InfiltrationClearing SolutionClearSeePBSSilicone FrameFluorescent Microscopy

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