Journal
/
Entwicklungsbiologie
/
Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells
JoVE Journal
Entwicklungsbiologie
Author Produced
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells

Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells

DOI:
10.3791/63348-v

•

08:09 min

•

August 29, 2022

•

, , ,
1Biochemistry Department, Faculty of Pharmacy,Ain Shams University, 2Pharmacology and Biochemistry Department, Faculty of Pharmacy,The British University in Egypt, 3Center for Drug Research and Development (CDRD), Faculty of Pharmacy,The British University in Egypt

Kapitel

  • 00:12Introduction
  • 01:35Isolation of Ad-MSCs from Rat Epididymal Fat Pads
  • 06:37Ergebnisse
  • 07:42Conclusions

Summary

Automatische Übersetzung

Automatische Übersetzung

Adipose tissue-derived mesenchymal stem cells (Ad-MSCs) can be a potential source of MSCs that differentiate into insulin-producing cells (IPCs). In this protocol, we provide detailed steps for the isolation and characterization of rat epididymal Ad-MSCs, followed by a simple, short protocol for the generation of IPCs from the same rat Ad-MSCs.

Tags

Mesenchymal Stem CellsIsolationCharacterizationDifferentiationInsulin-producing CellsCollagenase DigestionCD MarkersMesodermal Lineages

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin

Single-cell Photoconversion in Living Intact Zebrafish

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Read Article