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Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay

Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay

DOI:
10.3791/63187-v

•

06:49 min

•

January 12, 2022

•

,
1Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas,Universidad Nacional Autónoma de México

Kapitel

  • 00:04Introduction
  • 00:48Egg Incubation and Embryo Staging
  • 02:08Bead Preparation
  • 04:34Embryo Manipulation and Interdigit Implantation
  • 05:42Results: Evaluation of Cell Behavior in the Embryonic Chick Limb Using Soaked Beads
  • 06:20Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The soaked bead assay involves targeted delivery of test reagent at any developmental time point to study the regulation of cell differentiation and morphogenesis. A detailed protocol, applicable to any experimental animal model, for preparing three different types of soaked beads and implanting these in the interdigit of a chicken embryo is presented.

Tags

Cell DifferentiationMorphogenesisPatterningChicken EmbryogenesisSoaked-bead AssayEntwicklungsbiologieEmbryoHamburger-Hamilton StagesChorioallantoic MembraneAffi-gel Heparin BeadsPBSIn Ovo

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