Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Isolation of Murine Retinal Endothelial Cells for …
JoVE Journal
Developmental Biology

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Developmental Biology
Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing
 

Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing

Article DOI: 10.3791/63133-v • 09:59 min • October 11th, 2021
October 11th, 2021 •
1,2, 1, 2,3,4, 1,2,4,5
1Department of Cell Biology, University of Virginia School of Medicine, 2Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine, 3Department of Cardiology, University of Virginia School of Medicine, 4Hematovascular Biology Center, University of Virginia School of Medicine, 5Yale Cardiovascular Research Center, Yale University School of Medicine

Kapitel

Summary
Automatic Translation

This protocol describes a method for the isolation of murine postnatal retinal endothelial cells optimized for cell yield, purity, and viability. These cells are suitable for next-generation sequencing approaches.

Tags

Retinal Endothelial Cells Next-generation Sequencing Isolation Method Vascular Development Viability Purity Sequencing Techniques Shelby Cain Graduate Student Hershey Laboratory Neonatal Mouse Eye Removal Dissection Scissors Forceps Optic Nerve 48-well Plate PBS Retinal Tissue Isolation Dissection Pad Petri Dish Transfer Pipe Pad Dissection Microscope Anterior Chamber Cornea Sclera
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter