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Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
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Kapitel
- 00:05Introduction
- 00:55Optimization of Scanning Confocal Microscope Settings
- 01:58Live Imaging of the Mitochondrial Redox Response to N-methyl-D-aspartate (NMDA) Treatment
- 03:08Data Analysis
- 05:40Results: Effects of N-methyl-D-aspartate (NMDA) on Neuronal Mitochondria Oxidation
- 07:04Conclusion
This article describes a protocol to determine differences in basal redox state and redox responses to acute perturbations in primary hippocampal and cortical neurons using confocal live microscopy. The protocol can be applied to other cell types and microscopes with minimal modifications.
