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In vitro Flerparametrisk cellulær analyse af mikro organiske charge-moduleret Felt-effekt Transistor Arrays
JoVE Journal
Biotechnik
This content is Free Access.
JoVE Journal Biotechnik
In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays
DOI:

10:05 min

September 20, 2021

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Kapitel

  • 00:04Introduction
  • 01:12Substrate Selection, Preparation, and Floating Gates (FG): Titanium Deposition
  • 02:11FG Patterning and Gate Dielectric Deposition
  • 03:55Opening of the Sensing Areas of the OCMFET for Electrical Activity Recording and Formation of the Vias to Access the Back of the FGs
  • 04:46Self-Alignment of Source and Drain with the FG
  • 05:15Gold Deposition, Channel Formation, and Patterning of the Sources, Drains, and Control Gates
  • 06:50Deposition and Activation of the Parylene C for pH Sensing
  • 07:57Semiconductor Deposition, Culturing Chamber Placing, and Final Cut-Out of the Device from the PET
  • 08:22Results: Cellular Electrical and Metabolic Activity Recordings with Micro OCMFET Arrays (MOA)
  • 09:27Conclusion

Summary

Automatische Übersetzung

Her præsenterer vi fabrikationsprotokollen for en organisk charge-moduleret felteffekttransistor (OCMFET)-baseret enhed til in vitro cellulær interfacing. Enheden, kaldet en mikro OCMFET array, er en fleksibel, billig, og reference-mindre enhed, som vil gøre det muligt at overvåge de elektriske og metaboliske aktiviteter af elektroaktive cellekulturer.

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