Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Preparation of Pseudo-Typed H5 Avian Influenza…
JoVE Journal
Immunology and Infection

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Immunology and Infection
Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity
 

Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity

Article DOI: 10.3791/62626-v • 07:15 min • November 22nd, 2021
November 22nd, 2021 •
*1, *1, 1,2, 3, 2, 3, 4, 1
1Nanjing Advanced Academy of Life and Health, 2Institute of Animal Bio-technology, Jilin Academy of Agricultural Sciences, 3National Clinical Research Center for Infectious Diseases, Shenzhen Third People’s Hospital, Southern University of Science and Technology, 4CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences
* These authors contributed equally

Kapitel

Summary
Automatic Translation

Here we describe a protocol for pseudovirus packaging and the measurement of antibody neutralizing activity.

Tags

Pseudo-Typed H5 Avian Influenza Viruses Calcium Phosphate Transfection Method Antibody Neutralizing Activity Pseudo Virus Preparations Pseudo Virus Neutralization Assays Troubleshooting Modifications HEK293 FT Cells Complete DMEM Medium Chloroquine Reagents Plasmid DNA Supernatant Hemagglutinin Protein Expression PBS 96 Well Round Bottom Plate
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter