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Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform
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1Department of Bioengineering and Aerospace Engineering,Universidad Carlos III de Madrid (UC3M), 2Group of Optics, Photonics and Biophotonics (GOFB). Center for Biomedical Technology,Universidad Politécnica de Madrid, 3Division of Epithelial Biomedicine,CIEMAT, 4Instituto de Investigación Sanitaria Gregorio Marañón
Kapitel
- 00:04Introduction
- 00:48Chip Design and Micromachining Parameters
- 01:44Chip Assembly
- 02:43Pump Connections and Parallel Flow Protocol
- 03:55Human Immortalized Skin Keratinocytes (hKCs) Monolayer Seeding
- 04:31Results: Characterization of Skin Model in The Microfluidic Chip with Confocal and Fluorescence Microscopy
- 05:49Conclusion
Here, we present a protocol to generate a three-dimensional simplified and undifferentiated skin model using a micromachined microfluidic platform. A parallel flow approach allows the in situ deposition of a dermal compartment for the seeding of epithelial cells on top, all controlled by syringe pumps.
