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Ensayo de imagen cuantitativa in vitro para la fagocitosis de células muertas de neuroblastoma por iPSC-Macrófagos

JoVE Journal
Neurowissenschaften

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JoVE Journal Neurowissenschaften

In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages

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Ensayo de imagen cuantitativa in vitro para la fagocitosis de células muertas de neuroblastoma por iPSC-Macrófagos

4,864 Views

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09:48 min

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February 14, 2021

DOI:

10.3791/62217-v

DOI:

10.3791/62217-v

•

09:48 min

February 14, 2021

•

1 Views

Hazel Hall-Roberts^2,3, Elena Di Daniel, William S. James, John B. Davis, Sally A. Cowley

¹James Martin Stem Cell Facility, Sir William Dunn School of Pathology,University of Oxford, ²Alzheimer’s Research UK Oxford Drug Discovery Institute, Nuffield Department of Medicine Research Building,University of Oxford, ³UK Dementia Research Institute,Cardiff University

Tags

In Vitro Quantitative Imaging Assay Phagocytosis Dead Neuroblastoma Cells IPSC-macrophages Neurodegenerative Disease SH-SY5Y Cells Cell Dissociation Fixation PH-sensitive Dye Fluorescent Dye Macrophage Media Hoechst 33342

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