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आईपीएससी-मैक्रोफेज द्वारा डेड न्यूरोब्लास्टोमा कोशिकाओं के फागोसिटोसिस के लिए इन विट्रो क्वांटिटेटिव इमेजिंग परख
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Neurowissenschaften
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JoVE Journal Neurowissenschaften
In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages
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आईपीएससी-मैक्रोफेज द्वारा डेड न्यूरोब्लास्टोमा कोशिकाओं के फागोसिटोसिस के लिए इन विट्रो क्वांटिटेटिव इमेजिंग परख

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09:48 min

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February 14, 2021

DOI:

10.3791/62217-v

DOI:
10.3791/62217-v

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09:48 min
February 14, 2021

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1James Martin Stem Cell Facility, Sir William Dunn School of Pathology,University of Oxford, 2Alzheimer’s Research UK Oxford Drug Discovery Institute, Nuffield Department of Medicine Research Building,University of Oxford, 3UK Dementia Research Institute,Cardiff University

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In VitroQuantitative Imaging AssayPhagocytosisDead Neuroblastoma CellsIPSC-macrophagesNeurodegenerative DiseaseSH-SY5Y CellsCell DissociationFixationPH-sensitive DyeFluorescent DyeMacrophage MediaHoechst 33342

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Hall-Roberts, H., Di Daniel, E., James, W. S., Davis, J. B., Cowley, S. A. In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages. J. Vis. Exp. (168), e62217, doi:10.3791/62217 (2021).

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