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면역 형광을 사용하여 제브라피시 배아 심장에서 PM2.5-유도 된 DNA 손상을 감지합니다.
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Using Immunofluorescence to Detect PM2.5-induced DNA Damage in Zebrafish Embryo Hearts
Please note that all translations are automatically generated. Click here for the English version.

면역 형광을 사용하여 제브라피시 배아 심장에서 PM2.5-유도 된 DNA 손상을 감지합니다.

3,456 Views

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05:58 min

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February 15, 2021

DOI:

10.3791/62021-v

DOI:
10.3791/62021-v

•

05:58 min
February 15, 2021

•

13 Views
, , , , , , ,
1School of Public Health,Soochow University, 2Toxicology, Risk Assessment and Research Division,Texas Commission on Environmental Quality

Summary

Automatically generated

이 프로토콜은 면역 형광 분석기를 사용하여 제브라피시 배아의 해부 된 심장에서 PM2.5-유도 된 DNA 손상을 감지합니다.

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ImmunofluorescencePM2.5DNA DamageZebrafish EmbryoHeart8-hydroxydeoxyguanosineGamma-H2AXAntibodyFixationImmunostainingMicroscopy

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Cite this Article

Huang, Y., Tao, Y., Cai, C., Chen, J., Ji, C., Aniagu, S., Jiang, Y., Chen, T. Using Immunofluorescence to Detect PM2.5-induced DNA Damage in Zebrafish Embryo Hearts. J. Vis. Exp. (168), e62021, doi:10.3791/62021 (2021).

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