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Neutron Crystallography Data Collection and Processing for Modelling Hydrogen Atoms in Protein Structures
Kapitel
- 00:04Introduction
- 00:56Crystal Harvesting and Mounting
- 02:28Room Temperature Data Collection
- 03:31Cryo Data Collection
- 04:36Structure Refinement
- 06:30Model Building
- 07:45Results: Representative Neutron Crystallography Data Collection and Processing
- 09:26Conclusion
Neutron protein crystallography is a structural technique that permits the localization of hydrogen atoms, thereby providing important mechanistic details of protein function. We present here the workflow for mounting a protein crystal, neutron diffraction data collection, structure refinement and analysis of the neutron scattering length density maps.
