Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Isolamento de Histone do tecido da folha de sorgo para perfil de espectrometria de massa de top down de potenciais marcadores epigenéticos
1Environmental Molecular Sciences Laboratory,Pacific Northwest National Laboratory, 2DOE-Joint Genome Institute,Lawrence Berkeley Laboratory, 3Kearney Agricultural Research and Extension Center,University of California Agriculture and Natural Resources, 4West Side Research and Extension Center,University of California, 5Department of Plant Sciences,University of California, Davis, 6Department of Plant and Microbial Biology,University of California, Berkeley
Kapitel
- 00:05Introduction
- 00:40Nuclei Isolation from Sorghum Leaf Sample
- 02:13Isolation of Nuclei from Remaining Cytoplasmic Contaminants
- 03:05Nuclei Lysis and Histone Extraction
- 04:39Results: Qualitative & Quantitative Analysis of the Identified Histones
- 06:36Conclusion
O protocolo foi desenvolvido para extrair efetivamente histonas intactas de materiais de folhas de sorgo para o perfil de modificações pós-translacionais de histona que podem servir como potenciais marcadores epigenéticos para ajudar as culturas resistentes à seca de engenharia.
