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Sondare la cinetica dell'mRNA nello spazio e nel tempo in Escherichia coli utilizzando la fluorescenza a singola molecola a due colori in ibridazione situ
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July 30, 2020
DOI:
1Department of Physics,University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells,University of Illinois at Urbana-Champaign, 3Carl R. Woese Institute for Genomic Biology,University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign
Summary
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Questo protocollo descrive un'applicazione di fluorescenza a singola molecola in ibridazione situ (smFISH) per misurare la cinetica in vivo della sintesi e della degradazione dell'mRNA.
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Kim, S., Vaidya, K. Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization. J. Vis. Exp. (161), e61520, doi:10.3791/61520 (2020).
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