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Dissection, Immunohistochemistry and Mounting of Larval and Adult Drosophila Brains for Optic Lobe Visualization
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Kapitel
- 00:00Introduction
- 00:35Larval Brain Dissection
- 02:23Adult Brain Dissection
- 04:03Mounting Larval Brains
- 06:06Mounting Adult Brains
- 08:02Representative Images of Larval and Adult Optic Lobes
- 10:47Conclusion
This protocol describes three steps to prepare larval and adult Drosophila optic lobes for imaging: 1) brain dissections, 2) immunohistochemistry and 3) mounting. Emphasis is placed on step 3, as distinct mounting orientations are required to visualize specific optic lobe structures.
