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Journal
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Entwicklungsbiologie
/
为早期小鼠胚胎测序准备小型RNA库
JoVE Journal
Entwicklungsbiologie
This content is Free Access.
JoVE Journal
Entwicklungsbiologie
Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
Please note that all translations are automatically generated.
Click here for the English version.
为早期小鼠胚胎测序准备小型RNA库
DOI:
10.3791/61086-v
•
08:37 min
•
October 09, 2020
•
Rachel A. Keuls
,
Ronald Parchem
1
Development, Disease Models & Therapeutics Graduate Program, Department of Molecular and Cellular Biology, Department of Neuroscience, Center for Cell and Gene Therapy, Stem Cells and Regenerative Medicine Center
,
Baylor College of Medicine
Kapitel
00:04
Introduction
01:00
Embryo Dissection
01:51
Embryo Dissociation and Cell Sorting
03:11
Size Selection
06:02
Results: Small RNA Libraries
07:53
Conclusion
Summary
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Automatische Übersetzung
我们描述了一种在早期小鼠胚胎中分析微RNA的技术。该协议克服了低细胞输入和小RNA富集的挑战。此测定可用于分析早期小鼠胚胎不同细胞系中 miRNA 表达随时间变化。
Tags
Small RNA Sequencing
MicroRNAs
Embryonic Development
Low Input Samples
Embryo Dissection
Embryo Staging
Cell Suspension
Cell Sorting
RNA Extraction
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