Journal
/
Entwicklungsbiologie
/
A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons

A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons

DOI:
10.3791/60955-v

•

07:41 min

•

August 06, 2020

•

, , , ,
1Department of Molecular Genetics, Faculty of Biological Sciences,Tarbiat Modares University, 2Center for Therapeutic Innovation and Department of Psychiatry & Behavioral Sciences,University of Miami Miller School of Medicine, 3Department of Biochemistry and Molecular Biology,University of Miami Miller School of Medicine, 4Persian BayanGene Research and Training Center

Kapitel

  • 00:04Introduction
  • 00:51Passaging and Freezing the hNPCs
  • 02:50Image Acquisition and Analysis
  • 04:25Neurotoxicity Assessment
  • 06:10Results: Neurite Outgrowth Assay and Neurotoxicity Assessment with Small Molecule Compounds
  • 07:13Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The presented protocol describes a method for a neurite outgrowth assay and neurotoxicity assessment of small molecule compounds.

Tags

Keywords: Neurite Outgrowth AssayNeurotoxicity AssessmentHuman Neural Progenitor CellsNeuronsInduced Pluripotent Stem CellsEmbryonic Stem CellsNeurosphereCell DissociationCell CultureCryopreservationImage AnalysisFijiROI ManagerFreehand Line

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

check_url/de/60955?article_type=v

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells
check_url/de/60955?article_type=v

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord
check_url/de/60955?article_type=v

Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research
check_url/de/60955?article_type=v

Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration
check_url/de/60955?article_type=v

Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation
check_url/de/60955?article_type=v

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
check_url/de/60955?article_type=v

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks
check_url/de/60955?article_type=v

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
check_url/de/60955?article_type=v

A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes
check_url/de/60955?article_type=v

Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells

Read Article