Journal
/
Neurowissenschaften
/
Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches
JoVE Journal
Neurowissenschaften
This content is Free Access.
JoVE Journal Neurowissenschaften
Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches

Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches

DOI:
10.3791/60822-v

•

10:05 min

•

May 23, 2020

•

, , , , , , ,
1Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina,Universidade de Lisboa, 2Instituto de Farmacologia e Neurociências, Faculdade de Medicina,Universidade de Lisboa, 3Instituto de Biologia Molecular, Faculdade de Medicina,Universidade de Lisboa

Kapitel

  • 00:04Introduction
  • 00:59Postnatal Day 1-3 (P1-3) Mouse Brain Harvest
  • 02:28Subventricular Zone (SVZ)/Dentate Gyrus (DG) Microdissections
  • 04:22Tissue Dissociation
  • 05:27Postnatal Neural Stem Cell Expansion
  • 06:09Neurosphere Passaging
  • 07:10Results: Representative Neurosphere Expansion and Characterization
  • 09:21Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

In this article, we describe, in detail, a protocol for the generation of neurosphere cultures from postnatal mouse neural stem cells derived from the main mouse neurogenic niches. Neurospheres are used to identify neural stem cells from brain tissue allowing the estimation of precursor cell numbers. Moreover, these 3D structures can be plated in differentiative conditions, giving rise to neurons, oligodendrocytes and astrocytes, allowing the study of cell fate.

Tags

Neurosphere AssayNeural Stem/progenitor CellsAdult NeurogenesisBrain DisordersAlzheimer’sParkinson’sMultiple SclerosisPostnatal Mouse BrainSubventricular ZoneDentate GyrusMicrodissectionHBSSMeningesCerebellumOlfactory Bulbs

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice

Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone

Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains

Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains

Generation of Neurospheres from Mixed Primary Hippocampal and Cortical Neurons Isolated from E14-E16 Sprague Dawley Rat Embryo

Purification of Prominin-1+ Stem Cells from Postnatal Mouse Cerebellum

Culture of Neurospheres Derived from the Neurogenic Niches in Adult Prairie Voles

Read Article