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Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
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March 25, 2020
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Two methods of cholesterol enrichment are presented: the application of cyclodextrin saturated with cholesterol to enrich mammalian tissues and cells, and the use of cholesterol-enriched phospholipid-based dispersions (liposomes) to enrich Xenopus oocytes. These methods are instrumental for determining the impact of elevated cholesterol levels in molecular, cellular, and organ function.
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Slayden, A., North, K., Bisen, S., Dopico, A. M., Bukiya, A. N., Rosenhouse-Dantsker, A. Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol. J. Vis. Exp. (157), e60734, doi:10.3791/60734 (2020).
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