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Isolamento, cultura e induzione adipogenica di cellule staminali adipose originali adipose-derivate da cellule staminali periaortiche
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1Department of Cardiology, Shanghai Chest Hospital,Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences,Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension,Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine,Huadong Hospital Affiliated to Fudan University
Kapitel
- 00:04Introduction
- 01:24Periaortic Adipose Tissue (PAAT) Dissection
- 02:55Stromal Vascular Cell Fraction (SVCF) Isolation
- 04:51Neural Crest Original Adipose-Derived Stem Cell (NCADSC) Culture
- 06:13NCADSC Adipogenic Induction
- 06:42Results: Representative Adipocyte Differentiation and Characterization
- 07:58Conclusion
Vi presentiamo un protocollo per l'isolamento, la coltura e l'induzione adipogenica delle cellule staminali derivate dalla cresta neurale derivate da cellule staminali derivate da adiposo (NCADSC) dal tessuto adiposo periaortico del Wnt-1Cre Topi Rosa26RFP/z. Le NCADSC possono essere una fonte facilmente accessibile di ADSC per la modellazione dell'adipogenesi o della lipogenesi in vitro.
